Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Promoter polymorphisms in glutathione-S-transferase genes affect transcription

Guy, Carol, Hoogendoorn, Bastiaan ORCID:, Smith, Susan Kay, Coleman, Sharon Louise, O'Donovan, Michael Conlon ORCID: and Buckland, Paul Robert 2004. Promoter polymorphisms in glutathione-S-transferase genes affect transcription. Pharmacogenetics 14 (1) , pp. 45-51. 10.1097/00008571-200401000-00005

Full text not available from this repository.


The glutathione-S-transferases are a group of enzymes that play a major role in detoxification and defence against toxic, carcinogenic and other compounds. We analysed the proximal promoters of 14 genes encoding glutathione-S-transferase for polymorphism. Ten of the promoters contained sequence variants, nine of which we were able to clone into a reporter gene vector, pGL3. The relative ability of each haplotype to promote transcription of the luciferase gene was tested in each of two human cell lines (HEK293t and TE671) using a cotransfected CMV-SEAP plasmid as a control. Four genes (GSTA1, GSTA2, GSTM4 and GSTT2) showed activity differences greater than 1.5-fold between haplotypes, and a fifth gene (MGST1) showed a 1.4-fold difference. The promoter sequence variants in these genes may therefore play a role in human variation, susceptibility to diseases and the effects of drugs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Subjects: R Medicine > R Medicine (General)
ISSN: 0960-314X
Last Modified: 12 Dec 2022 08:55

Citation Data

Cited 41 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item