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A novel technique for assessment of adherence of Candida albicans to solid surfaces

Williams, David Wynne ORCID:, Waters, Mark Geoffrey John, Potts, Albert John Cornel and Lewis, Michael Alexander Oxenham ORCID: 1998. A novel technique for assessment of adherence of Candida albicans to solid surfaces. Journal of Clinical Pathology 51 (5) , pp. 390-391. 10.1136/jcp.51.5.390

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A novel approach for the assessment of adherence of Candida albicans to translucent acrylic material is described. The method uses the inverted microscope to visualise yeast adhering to acrylic surfaces while the test material remains immersed in buffer. Adherent cells were not subjected to surface tension forces that can occur during drying processes, so that an even distribution of yeast with no aggregation occurred. The process of counting attached yeast was subsequently performed without difficulty. From the 11 C albicans isolates examined, two groups were evident with respect to acrylic adherence: one group of four isolates with an adherence level of 400 yeast/mm2 acrylic, and one group of seven isolates with adherence levels of 1000 yeast/mm2 acrylic.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Candida albicans; adherence; acrylic.
Publisher: BMJ Journals
ISSN: 1472-4146
Last Modified: 25 Oct 2022 10:04

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