Combined ribosomal DNA and morphological analysis of individual gyrodactylid monogeneans

Harris, P. D., Cable, Joanne ORCID: https://orcid.org/0000-0002-8510-7055, Tinsley, R. C. and Lazarus, C. M. 1999. Combined ribosomal DNA and morphological analysis of individual gyrodactylid monogeneans. Journal of Parasitology 85 (2) , pp. 188-191. 10.2307/3285617

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Abstract

A method is presented for the isolation and analysis of hamuli, marginal hooks, and bars from individual gyrodactylid monogeneans using scanning electron microscopy (SEM), while simultaneously processing parasites for rDNA analysis using the polymerase chain reaction (PCR). The haptors of ethanol-fixed gyrodactylids were protease digested to liberate hooks for SEM, whereas DNA extracted from the bodies was used for PCR. The method resulted in hooks and hamuli being prepared from more than 90% of Gyrodactylus turnbulli individuals, a significant improvement on previously published digestion-based SEM techniques. PCR on the same parasites was less successful, but sequence data were obtained from 50% of individuals. Amplification of rDNA internal-transcribed spacer regions from individual worms used for SEM gave PCR products consistent with those predicted from our previous sequence analysis. This method allows the correlation of morphology and DNA sequence from the same individual and can be applied to ethanol-fixed material, such as field collected and museum specimens

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QL Zoology
Publisher:	JSTOR
ISSN:	0022-3395
Last Modified:	27 Oct 2022 08:18
URI:	https://orca.cardiff.ac.uk/id/eprint/61877

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