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EGF and FGF-2 responsiveness of rat and mouse neural precursors derived from the embryonic CNS

Kelly, Claire, Tyers, Pam, Borg, Melanie ter, Svendsen, Clive N., Dunnett, Stephen Bruce ORCID: and Rosser, Anne Elizabeth ORCID: 2005. EGF and FGF-2 responsiveness of rat and mouse neural precursors derived from the embryonic CNS. Brain Research Bulletin 68 (1-2) , pp. 83-94. 10.1016/j.brainresbull.2005.08.020

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EGF and FGF-2 induce the proliferation of embryonic neural precursors (ENPs) in vitro from a number of different species. In this study, we demonstrate that embryonic age is a crucial determinant of the number and differentiation potential of rat embryonic neural precursor cells responding to either EGF and/or FGF-2, in that (i) there is a differential response to the two growth factors (both alone and in combination) according to the gestational age of isolation and (ii) when allowed to differentiate, there are temporal changes in the ability of these cells to produce neurons. Furthermore, for cultures of all gestational ages, there is a defined pattern of senescence, with cultures expanding longest when cells are isolated earlier in gestation. The suggestion is that rat ENPs in this study consist predominantly of neural progenitor cells with limited division potential rather than self-renewing multipotential neural stem cells. In contrast, mouse ENPs appeared to expand indefinitely and thus allow for longer studies to be carried out looking at the effects of growth factor concentrations. The effect of varying the concentration of EGF was assessed using mouse ENPs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Neuroscience and Mental Health Research Institute (NMHRI)
Publisher: Elsevier
ISSN: 0361-9230
Last Modified: 27 Oct 2022 08:28

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