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The survival of neural precursor cell grafts is influenced by in vitro expansion

Zietlow, Rike, Pekarik, Vladimir ORCID:, Armstrong, Richard J. E., Tyers, Pamela, Dunnett, Stephen Bruce ORCID: and Rosser, Anne Elizabeth ORCID: 2005. The survival of neural precursor cell grafts is influenced by in vitro expansion. Journal of Anatomy 207 (3) , pp. 227-240. 10.1111/j.1469-7580.2005.00449.x

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Embryonic neural precursor cells (ENPs) provide a potential alternative for transplantation in neurodegenerative diseases, as they can be expanded in culture, avoiding many of the practical obstacles that limit the application of transplanting primary neurones. However, grafts of ENPs into animal models show variable survival and limited differentiation into neurones. The effect of expansion time on their ability to survive and differentiate may be an important factor in this and has not been examined directly. In these experiments, murine and human ENPs were expanded for short (4 weeks) and long (20 weeks) periods before transplantation into the adult rat striatum. Whereas grafts of both short- and long-term expanded human ENPs survived for 4 weeks following transplantation, by 20 weeks all long-term expanded grafts had disappeared. Murine ENPs behaved similarly: only grafts of short-term expanded ENPs survived at 12 weeks following transplantation. RT-PCR analysis of ENP cultures after 4 and 20 weeks of expansion demonstrated changes in expression of a number of different groups of genes. We conclude that long-term expansion of ENPs profoundly impairs their ability to survive long-term after transplantation into the adult brain. This has implications for the potential use of these cells for neural transplantation strategies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Neuroscience and Mental Health Research Institute (NMHRI)
Subjects: Q Science > QP Physiology
R Medicine > R Medicine (General)
Uncontrolled Keywords: human; murine; rat striatum; stem cells
Publisher: Wiley
ISSN: 0021-8782
Last Modified: 27 Oct 2022 08:28

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