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Microsphere-based tracing and molecular delivery in embryonic stem cells

Tsakiridis, Anestis, Alexander, Lois M., Gennet, Nicole, Sanchez-Martin, Rosario M., Livigni, Alessandra, Li, Meng ORCID:, Bradley, Mark and Brickman, Joshua M. 2009. Microsphere-based tracing and molecular delivery in embryonic stem cells. Biomaterials 30 (29) , pp. 5853-5861. 10.1016/j.biomaterials.2009.06.024

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Embryonic stem (ES) cells are in vitro cell lines that can differentiate into all lineages of the fetus and the adult. Despite the versatility of genetic manipulation in murine ES cells, these approaches are time-consuming and rely on inefficient transient cellular delivery systems that can only be applied to undifferentiated ES cell cultures. Here we describe a polystyrene microsphere-based system designed to efficiently deliver biological materials into both undifferentiated and differentiating ES cells. Our results demonstrate that these microspheres can be successfully employed for simultaneous cellular labeling and controlled transfer of various cargos such as fluorophores, proteins and nucleic acids into ES cells without any significant toxicity or loss of pluripotency. This versatile delivery system is also effective in other stem cell lines derived from early embryos, trophoblast and neural stem cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Embryonic stem cell; Differentiation; Transfection; Delivery; Microspheres.
Publisher: Elsevier
ISSN: 0142-9612
Last Modified: 27 Oct 2022 08:40

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