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What can we learn about cell signalling by combining optical imaging and patch clamp techniques?

Park, Myoung, Tepikin, Alexei V. and Petersen, Ole Holger 2002. What can we learn about cell signalling by combining optical imaging and patch clamp techniques? Pflugers Archiv: European Journal of Physiology 444 (3) , pp. 305-316. 10.1007/s00424-002-0832-y

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Optical imaging is a powerful technique with which to investigate the activity, distribution and movement of biomolecules. The increased resolution of images obtained with confocal microscopy now allows us to visualize the signalling events in individual intracellular organelles. Local photobleaching and uncaging of caged compounds enable investigators to control the activity of many biologically important molecules in small localized regions of both cytosol and internal spaces of cellular organelles. Uncaging and photobleaching conveniently complement laser scanning confocal microscopy. The whole-cell recording configuration of the patch-clamp technique has been widely used not only to measure ionic currents, but also to control the concentration of important molecules in the cytosol. The cell-attached configuration of patch clamp was utilized for local stimulation of the cell and local delivery of the second messengers. This paper describes the advantages of combining patch-clamp and optical imaging methods as well as some of the recent achievements using this approach.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Systems Immunity Research Institute (SIURI)
Uncontrolled Keywords: Caged compound Calcium; Confocal microscope; Fluorescence Imaging; Patch-clamp; Photobleaching Uncaging
Publisher: Springer
ISSN: 0031-6768
Last Modified: 04 Jun 2017 06:38

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