Context-dependent effects of charged residues in transmembrane segments of MalF–PhoA fusions

Ehrle, Rainer, Mikhaleva, Natasha, Boyd, Dana, Davidson, Amy L. and Ehrmann, Michael ORCID: https://orcid.org/0000-0002-1927-260X 2003. Context-dependent effects of charged residues in transmembrane segments of MalF–PhoA fusions. Research in Microbiology 154 (9) , pp. 654-657. 10.1016/j.resmic.2003.07.001

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Abstract

Charged residues were introduced into the C-terminal transmembrane segment of a MalF–PhoA fusion to investigate the efficiency of the altered transmembrane segment to function as an export signal or a stop transfer signal. PhoA assays revealed that charges had negative effects when the transmembrane segment was part of a stop transfer signal but not when it was part of an export signal. Implications of this finding for the biogenesis of polytopic membrane proteins are discussed.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Elsevier
ISSN:	0923-2508
Last Modified:	27 Oct 2022 08:46
URI:	https://orca.cardiff.ac.uk/id/eprint/63337

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