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Delayed methylation and the matrix bound DNA methylase

Davis, Terence, Kirk, David, Rinaldi, Angela, Burdon, Roy H. and Adams, Roger L. P. 1985. Delayed methylation and the matrix bound DNA methylase. Biochemical and Biophysical Research Communications 126 (2) , pp. 678-684. 10.1016/0006-291X(85)90238-4

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It is shown that the methylation of DNA that occurs in isolated nuclei is “delayed methylation”. This methylation is not reduced in nuclei which have been pretreated with 0.2M NaCl to extract the soluble methylase suggesting that this methylation is the product of a firmly bound matrix associated DNA methylase. Evidence is provided that, like the methylase, the DNA substrate is associated with the nuclear matrix.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QH Natural history > QH426 Genetics
R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0006-291X
Last Modified: 04 Jun 2017 06:41

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