Methylation of chromatin in vitro

Davis, Terence ORCID: https://orcid.org/0000-0003-2780-0262, Rinaldi, Angela, Clark, Lilian and Adams, Roger L. P. 1986. Methylation of chromatin in vitro. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 866 (4) , pp. 233-241. 10.1016/0167-4781(86)90048-5

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Abstract

The endogenous DNA methylase in nuclei isolated from growing mouse cells preferentially methylates DNA in micrococcal nuclease-resistant regions probably as a result of the location in these regions of the preponderance of hemimethylated sites. Added mouse ascites cell DNA methylase catalyses the methylation of exposed, nuclease-sensitive DNA in chromatin from growing or non-growing mouse or insect cells. The poor acceptor ability of nuclease-resistant regions in this situation is due to the presence of histone proteins which block de novo methylation. Transcriptionally active regions of chromatin are selectively methylated in vitro by either endogenous or added DNA methylase.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General)
Uncontrolled Keywords:	DNA methylation; DNA methylase; High-mobility-group protein; Non-histone protein; Chromatin; (Mouse)
Publisher:	Elsevier
ISSN:	0167-4781
Last Modified:	14 Dec 2022 07:28
URI:	https://orca.cardiff.ac.uk/id/eprint/63822

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