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Complete maturation of the plastid protein translocation channel requires a type I signal peptidase

Inoue, Kentaro, Baldwin, Amy Joy ORCID:, Shipman, Rebecca L., Matsui, Kyoko, Theg, Steven M. and Ohme-Takagi, Masaru 2005. Complete maturation of the plastid protein translocation channel requires a type I signal peptidase. Journal of cell biology 171 (3) , p. 425. 10.1083/jcb.200506171

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The protein translocation channel at the plastid outer envelope membrane, Toc75, is essential for the viability of plants from the embryonic stage. It is encoded in the nucleus and is synthesized with a bipartite transit peptide that is cleaved during maturation. Despite its important function, the molecular mechanism and the biological significance of the full maturation of Toc75 remain unclear. In this study, we show that a type I signal peptidase (SPase I) is responsible for this process. First, we demonstrate that a bacterial SPase I converted Toc75 precursor to its mature form in vitro. Next, we show that disruption of a gene encoding plastidic SPase I (Plsp1) resulted in the accumulation of immature forms of Toc75, severe reduction of plastid internal membrane development, and a seedling lethal phenotype. These phenotypes were rescued by the overexpression of Plsp1 complementary DNA. Plsp1 appeared to be targeted both to the envelope and to the thylakoidal membranes; thus, it may have multiple functions.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: Pdf uploaded in accordance with publisher's policy at (accessed 18.09.14).
Publisher: Rockefeller University Press,
ISSN: 0021-9525
Date of First Compliant Deposit: 30 March 2016
Last Modified: 21 May 2023 02:30

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