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Evidence for serca and BKCa activation in BNP protection of reperfused myocardium [Abstract]

Burley, Dwaine, George, Christopher, Wann, Kenneth Taylor and Baxter, Gary ORCID: 2008. Evidence for serca and BKCa activation in BNP protection of reperfused myocardium [Abstract]. Journal of Molecular and Cellular Cardiology 44 (4) , p. 717. 10.1016/j.yjmcc.2008.02.015

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B-type natriuretic peptide (BNP) given at the time of reperfusion, limits reperfusion-induced myocardial injury. Studies examining the endogenous components of this protection, have explored KATP channel activation, but no studies have focused on the sarcoplasmic reticulum Ca2+-ATPase (SERCA), or the large conductance K+(BKCa) channel. We hypothesised that BNP induced protection of reperfused myocardium involves SERCA and BKCa channel activation. Male rat hearts were Langendorff-perfused, and subjected to 35 min left coronary artery occlusion and 120 min reperfusion. Hearts were randomised to 15 min treatments, commencing 5 min prior to reperfusion with BNP, the SERCA inhibitors thapsigargin (TSG) and cyclopiazonic acid (CPA) (Study 1), or the BKCa channel activator NS1619 and inhibitor paxilline (PAX) (Study 2). Infarct size was measured using triphenyltetrazolium staining. These findings provide evidence for the involvement of two new post-receptor mechanisms, SERCA and BKCa activation, which are involved in BNP's protection of reperfused myocardium.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Ischaemia–reperfusion; Natriuretic peptides
Publisher: Elsevier
ISSN: 0022-2828
Last Modified: 17 Oct 2022 09:59

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