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A Comparative approach to understanding tissue-specific expression of uncoupling protein 1 expression in adipose tissue

Shore, Andrew, Emes, Richard D., Wessely, Frank, Kemp, Paul, Cillo, Clemente, D'Armiento, Maria, Hoggard, Nigel and Lomax, Michael A. 2013. A Comparative approach to understanding tissue-specific expression of uncoupling protein 1 expression in adipose tissue. Frontiers in Genetics 3 , 304. 10.3389/fgene.2012.00304

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The thermoregulatory function of brown adipose tissue (BAT) is due to the tissue-specific expression of uncoupling protein 1 (UCP1) which is thought to have evolved in early mammals. We report that a CpG island close to the UCP1 transcription start site is highly conserved in all 29 vertebrates examined apart from the mouse and xenopus. Using methylation sensitive restriction digest and bisulfite mapping we show that the CpG island in both the bovine and human is largely un-methylated and is not related to differences in UCP1 expression between white and BAT. Tissue-specific expression of UCP1 has been proposed to be regulated by a conserved 5′ distal enhancer which has been reported to be absent in marsupials. We demonstrate that the enhancer, is also absent in five eutherians as well as marsupials, monotremes, amphibians, and fish, is present in pigs despite UCP1 having become a pseudogene, and that absence of the enhancer element does not relate to BAT-specific UCP1 expression. We identify an additional putative 5′ regulatory unit which is conserved in 14 eutherian species but absent in other eutherians and vertebrates, but again unrelated to UCP1 expression. We conclude that despite clear evidence of conservation of regulatory elements in the UCP1 5′ untranslated region, this does not appear to be related to species or tissues-specific expression of UCP1.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. This document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission
Publisher: Frontiers Media
ISSN: 1664-8021
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 06:51

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