Cloning and sequencing of a full-length sea bream (Sparus aurata) β-actin cDNA

Santos, Cecilia R. A., Power, Deborah M., Kille, Peter ORCID: https://orcid.org/0000-0001-6023-5221, Llewellyn, Lynda, Ramsurn, Vimi, Wigham, Trevor and Sweeney, Glen E. 1997. Cloning and sequencing of a full-length sea bream (Sparus aurata) β-actin cDNA. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 117 (2) , pp. 185-189. 10.1016/S0305-0491(96)00328-8

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Abstract

A full-length cDNA clone encoding beta-actin (β-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea bream β-actin sequence showed 98% identity to carp and human β-actin and 95% and 94% identity to sea squirt and Dictyostelium cytoplasmic actins, respectively.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Elsevier
ISSN:	1096-4959
Last Modified:	27 Oct 2022 09:24
URI:	https://orca.cardiff.ac.uk/id/eprint/65597

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