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Human neutrophils synthesize IL-8 in an IgE-mediated activation

Monteseirin, J., Chacon Fernandez, Pedro, Vega, A., El Bekay, R., Alvarez, M., Alba, G., Conde, J., Pintado, E., Bedoya, F. and Sobrino, F. 2004. Human neutrophils synthesize IL-8 in an IgE-mediated activation. Journal of Leukocyte Biology 76 (3) , pp. 692-700. 10.1189/jlb.0903441

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It has been demonstrated that neutrophils are responsible for the release of large amounts of the inflammatory chemokine interleukin-8 (IL-8), associated with inflammation. To further define the mechanisms implicated, we have analyzed the response of human neutrophils from allergic patients to specific antigens or challenge with anti-immunoglobulin (Ig)E antibodies. Neutrophils showed a dose- and time-dependent production of IL-8. The release of the cytokine was parallel to expression of IL-8 mRNA analyzed by the polymerase chain reaction. This expression was transient—it occurred after 3 h of anti-IgE treatment and was maintained for 18 h. Trifluoperazine, EGTA, reduced nicotinamide adenine dinucleotide phosphate-oxidase inhibitors, and reactive oxygen species (ROS) scavengers inhibited IL-8 production, indicating a critical dependence of calcium and oxidative stress. Moreover, an inhibitory effect of cyclosporin A, an immunosuppressor that inhibits calcineurin activity, on IL-8 release and IL-8 mRNA expression was observed. This is the first evidence of the involvement of ROS and calcium/calcineurin in IgE-dependent IL-8 production. These findings open new perspectives into the functional role of neutrophils in IgE-associated diseases.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: JLB
ISSN: 07415400
Last Modified: 28 Jun 2019 02:02

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