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Specific allergens enhance elastase release in stimulated neutrophils from asthmatic patients

Monteseirín, Javier, Bonilla, I., Camacho, M. J., Chacon Fernandez, Pedro, Vega, Antonio, Chaparro, Antonio, Conde, J. and Sobrino, Francisco 2003. Specific allergens enhance elastase release in stimulated neutrophils from asthmatic patients. International Archives of Allergy and Immunology 131 (3) , pp. 174-181. 10.1159/000071483

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Background: The presence of the three forms of IgE receptor – the heterotrimeric high-affinity receptor for IgE (FcεRI), the low-affinity receptor for IgE (FcεRII/CD23) and the Mac-2/IgE-binding protein (εBP) – has been demonstrated on human neutrophils. We have previously shown that specific allergens are able to activate functional responses by neutrophils from allergic patients sensitized to those allergens. Neutrophils are present at the sites of allergic inflammation. The primary (azurophilic) granules of neutrophils contain a variety of enzymes, such as elastase, that might potentiate inflammation. It is not known whether specific allergens are able to elicit elastase release by neutrophils from allergic patients. In addition, we attempted to evaluate the relationship between neutrophil degranulation and lung function of the patients, measured as FEV1. Methods: Neutrophils were challenged in vitro with the specific allergens that produced clinical symptoms in asthmatic patients. The cells were also challenged with allergen to which the patients were not sensitive. Neutrophils from normal subjects were challenged with allergens as control. Results: The in vitro challenge of neutrophils with allergens to which the patients were sensitive elicited a release of elastase by these cells. The in vitro activation of neutrophils was highly allergen specific; allergens other than those accounting for clinical symptoms did not evoke elastase release, and allergens were ineffective on neutrophils from healthy donors. A significant inverse correlation was observed between elastase release and patients’ lung function, measured as FEV1. Conclusion: An IgE-dependent mechanism might promote elastase release by neutrophils at allergic sites. There is a significant inverse relationship between levels of elastase released by neutrophils from allergic patients and lung function, as assessed by FEV1.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Karger
ISSN: 1423-0097
Last Modified: 28 Jun 2019 02:02

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