A 115 kDa calmodulin-binding protein is located in rat liver endosome fractions

Enrich, C., Bachs, O. and Evans, William Howard 1988. A 115 kDa calmodulin-binding protein is located in rat liver endosome fractions. Biochemical Journal 255 (3) , pp. 999-1005.

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Abstract

The distribution of calmodulin-binding polypeptides in various rat liver subcellular fractions was investigated. Plasma-membrane, endosome, Golgi and lysosome fractions were prepared by established procedures. The calmodulin-binding polypeptides present in the subcellular fractions were identified by using an overlay technique after transfer from gels to nitrocellulose sheets. Distinctive populations of calmodulin-binding polypeptides were present in all the fractions examined except lysosomes. A major 115 kDa calmodulin-binding polypeptide of pI 4.3 was located to the endosome subfractions, and it emerges as a candidate endosome-specific protein. Partitioning of endosome fractions between aqueous and Triton X-114 phases indicated that the calmodulin-binding polypeptide was hydrophobic. Major calmodulin-binding polypeptides of 140 and 240 kDa and minor polypeptides of 40-60 kDa were present in plasma membranes. The distribution of calmodulin in the various endosome and plasma-membrane fractions was also analysed, and the results indicated that the amounts were high compared with those in the cytosol.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General) R Medicine > RZ Other systems of medicine
Publisher:	Biochemical Society
ISSN:	0264-6021
Related URLs:	http://www.biochemj.org/bj/default.htm
Last Modified:	17 Mar 2021 02:59
URI:	https://orca.cardiff.ac.uk/id/eprint/67119

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