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Adsorption and interactions of dentine phosphoprotein with hydroxyapatite and collagen

Milan, Anna Margaret, Sugars, Rachael V., Embery, Graham and Waddington, Rachel J. ORCID: 2006. Adsorption and interactions of dentine phosphoprotein with hydroxyapatite and collagen. European Journal of Oral Sciences 114 (3) , pp. 223-231. 10.1111/j.1600-0722.2006.00347.x

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Dentine phosphoprotein (DPP) has been proposed to both promote and inhibit mineral deposition during dentinogenesis. The present study aimed to investigate the molecular interactions of DPP and dephosphorylated DPP (DPP-p) with hydroxyapatite (HAP). Bovine DPP was purified and dephosphorylated by alkaline phosphatase to obtain DPP-p. DPP and DPP-p adsorption to HAP was determined along with their ability, when free in solution or bound to collagen, to influence HAP-induced crystal growth. Absorption isotherms suggested that lower DPP concentrations (1.5–6.25 µg ml?1) demonstrated a reduced affinity for HAP compared with higher protein concentrations (12.5–50.0 µg ml?1). Dephosphorylated DPP had a much reduced affinity for HAP compared with DPP. Dentine phosphoprotein inhibited seeded HAP crystal growth, in a dose-dependent manner, whilst removal of the phosphate groups reduced this inhibition. When bound to collagen fibrils, DPP significantly promoted the rate of HAP crystal growth over 0–8 min. Conversely, DPP-p and collagen significantly decreased the rate of crystal growth over 0–18 min. These results indicate a major role for the phosphate groups present on DPP in HAP crystal growth. In addition, concentration-dependent conformational changes to DPP, and the interaction with other matrix components, such as collagen, are important in predicting its dual role in the mineralization of dentine.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: dentine ; hydroxyapatite ; mineralization ; phosphoprotein
Publisher: Wiley-Blackwell
ISSN: 0909-8836
Last Modified: 17 Oct 2022 08:39

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