Bidon, Tobias, Frosch, Christiane, Eiken, Hans G., Kutschera, Verena E., Hagen, Snorre B., Aarnes, Siv G., Fain, Steven R., Janke, Axel and Hailer, Frank ![]() |
Abstract
We report a new approach for molecular sex identification of extant Ursinae and Tremarctinae bears. Two Y-specific fragments (SMCY and 318.2) and one X-specific fragment (ZFX) are amplified in a multiplex PCR, yielding a double test for male-specific amplification and an internal positive control. The primers were designed and tested to be bear-specific, thereby minimizing the risk of cross-amplification in other species including humans. The high sensitivity and small amplicon sizes (100, 124, 160 base pairs) facilitate analysis of non-invasively obtained DNA material. DNA from tissue and blood as well as from 30 non-invasively collected hair and faeces yielded clear and easily interpretable results. The fragments were detected both by standard gel electrophoresis and automated capillary electrophoresis.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences |
Subjects: | Q Science > QL Zoology |
Uncontrolled Keywords: | bears; molecular sexing; multiplex PCR; non-invasive; Ursidae ; Ursus arctos |
Publisher: | Wiley-Blackwell |
ISSN: | 1755-098X |
Last Modified: | 27 Oct 2022 10:23 |
URI: | https://orca.cardiff.ac.uk/id/eprint/69925 |
Citation Data
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