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Elevations in cytosolic free Ca2+ are not required to trigger apoptosis in human leukaemia cells

Lennon, S. V., Ilfeather, S. A. K., Hallett, Maurice Bartlett ORCID:, Campbell, A. K. and Cotter, T. G. 2008. Elevations in cytosolic free Ca2+ are not required to trigger apoptosis in human leukaemia cells. Clinical and Experimental Immunology 87 (3) , pp. 465-471. 10.1111/j.1365-2249.1992.tb03021.x

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Previous studies have indicated that Ca2+ is a trigger Tor apoptosis (programmed cell death) in thymocytes and related cell lines. Recently we have shown that levels of apoptosis in leukacmic cells are diminished in Ca2+-deficient conditions, indicating that Ca2+ may be important in the mechanism of apoptosis in these cells. In the present study we investigated the possibility that Ca2+ serves as a trigger for apoptosis in the human leukaemic cell line, HL-60. Using fura-2 to measure cytosolic free Ca2+ concentrations, [Ca2+]i, in cell suspensions, and by using ratio imaging of fura-2 in single cells, we did not observe an early significant increase in [Ca2+]f, in HL-60 cells undergoing apoptosis. The latter stages of apoptosis were, however, accompanied by increasing [Ca2+]f, these increases were apparently a result of, rather than a cause of, apoptosis. Furthermore, apoptosis could be induced in HL-60 cells under conditions of vastly reduced [Ca2+]i achieved by loading these cells with fura-2 in the presence of EGTA. These results indicate that elevation of [Ca2+]i is not a prerequisite for apoptosis in HL-60 cells and that apoptosis can occur in these cells in the presence of low[Ca2+].

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Wiley-Blackwell
ISSN: 0009-9104
Last Modified: 28 Oct 2022 09:01

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