Pettit, E. J. and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 1998. Ca2+ signalling delays in neutrophils: effects of prior exposure to platelet activating factor or formyl-met-leu-phe. Cellular Signalling 10 (1) , pp. 49-53. 10.1016/S0898-6568(97)00073-9 |
Abstract
Rapid-time confocal scanning of fluo3-loaded neutrophils revealed that in individual cells there were grossly heterogeneous time intervals between stimulation with either f-met-leu-phe or platelet activating factor (PAF) and the initiation of Ca2+ influx, ranging from 75 msec to several seconds. The distribution of lag times after stimulation with f-met-leu-phe (100 nM) was influenced by prior stimulation with either f-met-leu-phe or PAF. However, whereas prior stimulation with f-met-leu-phe (50 nM) caused the subsequent cytosolic free Ca2+ response to second challenge with f-met-leu-phe to be delayed, prior stimulation with PAF (100 nM) caused an increase in the rapidity of the onset of the second response to f-met-leu-phe. With both stimuli, the cytosolic free Ca2+ in some neutrophils (non-Ca2+ responders) in the population did not increase significantly. However, some of these cells responded to the subsequent challenge. However, with both pre-treatment stimuli, those cells in which a significant Ca2+ response was provoked by the first stimulus, responded significantly faster than the initial `non-Ca2+ responders.' However, the reduced lag time provoked by pre-stimulation was not inhibited in neutrophils in which cytosolic free Ca2+ changes were dampened by intracellular BAPTA. These data point to post-receptor events, other than prior cytosolic free Ca2+ elevation, being important in determining the response Ca2+ delay to subsequent stimulation.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Subjects: | R Medicine > R Medicine (General) |
Publisher: | Elsevier |
ISSN: | 0898-6568 |
Last Modified: | 28 Oct 2022 09:03 |
URI: | https://orca.cardiff.ac.uk/id/eprint/73121 |
Citation Data
Cited 5 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
Edit Item |