Spatial and temporal separation of calcium signals in myeloid cells stimulated by immune complexes

Davies, E. V., Roberts, G. M. and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 1997. Spatial and temporal separation of calcium signals in myeloid cells stimulated by immune complexes. Cellular Signalling 9 (6) , pp. 457-462. 10.1016/S0898-6568(97)00039-9

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Abstract

Stimulation of large (100 μm) human myeloid cells with immune complexes resulted in Ca2+ spiking. Both global and regional changes in the intracellular cytosolic free Ca2+ concentration were detected in response to immune complex stimulation. The regional changes were mediated by release of Ca2+ from stores, whereas global changes were mediated by Ca2+ influx. They occurred independently of each other, with release of Ca2+ from intracellular Ca2+ stores being separated from transmembrane influx of Ca2+. Bromophenacyl bromide, an l-plastin binding agent, inhibited store release without preventing transmembrane influx of Ca2+. The large size of the myeloid cells used here allowed the visualisation of the spatial and temporal separation of store release from transmembrane influx of Ca2+, providing further evidence for the existence of independent Ca2+ store release and Ca2+ influx mechanisms in these cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Elsevier
ISSN:	0898-6568
Last Modified:	28 Oct 2022 09:03
URI:	https://orca.cardiff.ac.uk/id/eprint/73123

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