Localised and global cytosolic Ca2+ changes in neutrophils during engagement of Cd11b/CD18 integrin visualised using confocal laser scanning reconstruction

Pettit, E. J. and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 1996. Localised and global cytosolic Ca2+ changes in neutrophils during engagement of Cd11b/CD18 integrin visualised using confocal laser scanning reconstruction. Journal of Cell Science 109 (7) , pp. 1689-1694.

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Abstract

A confocal laser scanning technique was used to provide optical sections in the vertical plane through living neutrophils during engagement of integrin and shape change. This has permitted the visualisation of cytosolic free Ca2+ rises localised to the sites of integrin immobilisation. These localised Ca2+ changes resulted from the release of Ca2+ from intracellular stores, and were not inhibited by removal of extracellular Ca2+ or by the Ca2+ channel blocking nickel ions. After integrin engagement and initial cell shape changes, a second rapid phase of cell spreading occurred, which was accompanied by global Ca2+ signalling bursts that continued sporadically after maximum spreading. This global signalling was driven mainly by Ca2+ influx from the extracellular medium. We propose that the localised and global Ca2+ signalling triggered by integrin engagement results from a common underlying mechanism and these signals are important for neutrophil shape change and extravasation

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Antigens, CD11/analysis, Antigens, CD18/analysis, Calcium/metabolism*, Cells, Cultured, Humans, Microscopy, Confocal, Neutrophil Activation, Neutrophils/metabolism, Signal Transduction
Publisher:	The Company of Biologists Ltd
ISSN:	0021-9533
Last Modified:	28 Oct 2022 09:04
URI:	https://orca.cardiff.ac.uk/id/eprint/73138

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