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Development of a sporicidal test method for Clostridium difficile

Fraise, A. P., Wilkinson, M. A. C., Bradley, C. R., Paton, S., Walker, J., Maillard, J.-Y. ORCID:, Wesgate, R., Hoffman, P., Coia, J., Woodall, C., Fry, C. and Wilcox, M. 2015. Development of a sporicidal test method for Clostridium difficile. Journal of Hospital Infection 89 (1) , pp. 2-15. 10.1016/j.jhin.2014.09.014

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Background Disinfectants with claimed activity against Clostridium difficile must be evaluated to ensure efficacy against the spores that comprise an environmental source of patient infection. Unfortunately there is, at present, no generally accepted method for evaluating these disinfectants. In the absence of such a method, laboratories have to adapt protocols that were not designed for products used in medical environments and consequently may use inappropriate test organisms, exposure times, and pass criteria. Aim To develop and evaluate a method for testing the activity of disinfectants against C. difficile spores using exposure times and pass criteria which are relevant to clinical application. Methods A Joint Working Party of the Healthcare Infection Society (HIS) and the Advisory Committee on Antimicrobial Resistance and Healthcare Associated Infections (ARHAI) of the Department of Health in England was assembled. The Working Party adapted a previously described enzyme-based method for spore purification (the Clospore method) using an exposure time of 5 min and a 5 log10 kill as a pass criterion. Findings Evaluation of the method by three laboratories demonstrated that the method is simple to follow and that the results are repeatable and reproducible. Conclusion The method described by the Working Party produces a clean suspension with a high titre of spores. It is recommended that, for a disinfectant used in the environment, the product should demonstrate a 5 log10 reduction in 5 min under clean or dirty conditions to fulfil the requirements of the test.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords: Clostridium difficile; Disinfectant; Testing
Additional Information: Available online 24 October 2014
Publisher: Elsevier
ISSN: 0195-6701
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 22 September 2014
Last Modified: 28 Oct 2022 09:41

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