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Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice: a 24-hour and 28-day follow-up study

Chuang, Hsiao-Chi, Pan, Chih-Hong, Chuang, Kai-Jen, Chen, Jen-Kun, Hsiao, Ta-Chih, Lai, Ching-Huang, Jones, Timothy Peter, Berube, Kelly Ann, Hong, Gui-Bing and Ho, Kin-Fai 2015. Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice: a 24-hour and 28-day follow-up study. International Journal of Nanomedicine 10 , pp. 4705-4716. 10.2147/IJN.S82979

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Although zinc oxide nanoparticles (ZnONPs) are recognized to cause systemic disorders, little is known about the mechanisms that underlie the time-dependent differences that occur after exposure. The objective of this study was to investigate the mechanistic differences at 24 hours and 28 days after the exposure of BALB/c mice to ZnONPs via intratracheal instillation. An isobaric tag for the relative and absolute quantitation coupled with liquid chromatography/tandem mass spectrometry was used to identify the differential protein expression, biological processes, molecular functions, and pathways. A total of 18 and 14 proteins displayed significant changes in the lung tissues at 24 hours and 28 days after exposure, respectively, with the most striking changes being observed for S100-A9 protein. Metabolic processes and catalytic activity were the main biological processes and molecular functions, respectively, in the responses at the 24-hour and 28-day follow-up times. The glycolysis/gluconeogenesis pathway was continuously downregulated from 24 hours to 28 days, whereas detoxification pathways were activated at the 28-day time-point after exposure. A comprehensive understanding of the potential time-dependent effects of exposure to ZnONPs was provided, which highlights the metabolic mechanisms that may be important in the responses to ZnONP.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Earth and Environmental Sciences
Subjects: Q Science > QR Microbiology
Uncontrolled Keywords: glycolysis, iTRAQ, metabolism, nanoparticles, S100-A9
Publisher: Dove Medical Press
ISSN: 1178-2013
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 6 May 2015
Last Modified: 21 Mar 2019 00:13

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