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Measuring the refractive index of bovine corneal stromal cells using quantitative phase imaging

Gardner, Steven J., White, Nick, Albon, Julie ORCID:, Knupp, Carlo ORCID:, Kamma-Lorger, Christina S. and Meek, Keith M. ORCID: 2015. Measuring the refractive index of bovine corneal stromal cells using quantitative phase imaging. Biophysical Journal 109 (8) , pp. 1592-1599. 10.1016/j.bpj.2015.08.046

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The cornea is the primary refractive lens in the eye and transmits >90% of incident visible light. It has been suggested that the development of postoperative corneal haze could be due to an increase in light scattering from activated corneal stromal cells. Quiescent keratocytes are thought to produce crystallins that match the refractive index of their cytoplasm to the surrounding extracellular material, reducing the amount of light scattering. To test this, we measured the refractive index (RI) of bovine corneal stromal cells, using quantitative phase imaging of live cells in vitro, together with confocal microscopy. The RI of quiescent keratocytes (RI = 1.381 ± 0.004) matched the surrounding matrix, thus supporting the hypothesis that keratocyte cytoplasm does not scatter light in the normal cornea. We also observed that the RI drops after keratocyte activation (RI = 1.365 ± 0.003), leading to a mismatch with the surrounding intercellular matrix. Theoretical scattering models showed that this mismatch would reduce light transmission in the cornea. We conclude that corneal transparency depends on the matching of refractive indices between quiescent keratocytes and the surrounding tissue, and that after surgery or wounding, the resulting RI mismatch between the activated cells and their surrounds significantly contributes to light scattering.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Publisher: Biophysical Society
ISSN: 0006-3495
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 27 August 2015
Last Modified: 11 Oct 2023 20:55

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