Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils

Hallett, Maurice ORCID: https://orcid.org/0000-0001-8197-834X, Al-Jumaa, Maha and Dewitt, Sharon ORCID: https://orcid.org/0000-0001-8169-8241 2014. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. Quinn, Mark T. and DeLeo, Frank R., eds. Neutrophil Methods and Protocols, Methods in Molecular Biology, vol. 1124. Humana Press, pp. 107-120. (10.1007/978-1-62703-845-4_8)

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Abstract

The measurement and manipulation of cytosolic free Ca2+ of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca2+ signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils and are discussed here, especially the use of fluorescent indicators of Ca2+ and photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Dentistry Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Humana Press
ISBN:	978-1-62703-845-4
ISSN:	10643745
Last Modified:	28 Oct 2022 10:36
URI:	https://orca.cardiff.ac.uk/id/eprint/78963

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