Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca2+ oscillations required for mammalian oocyte activation

Nomikos, Michail, Theodoridou, Maria, Elgmati, Khalil, Parthimos, Dimitris ORCID: https://orcid.org/0000-0003-3852-323X, Calver, Brian L., Buntwal, Luke, Nounesis, George, Swann, Karl ORCID: https://orcid.org/0000-0002-4355-1449 and Lai, F. Anthony ORCID: https://orcid.org/0000-0003-2852-8547 2014. Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca2+ oscillations required for mammalian oocyte activation. Molecular Human Reproduction 20 (6) , pp. 489-498. 10.1093/molehr/gau011

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Abstract

A sperm-specific phospholipase C-zeta (PLCζ) is believed to play an essential role in oocyte activation during mammalian fertilization. Sperm PLCζ has been shown to trigger a prolonged series of repetitive Ca2+ transients or oscillations in oocytes that precede activation. This remarkable intracellular Ca2+ signalling phenomenon is a distinctive characteristic observed during in vitro fertilization by sperm. Previous studies have notably observed an apparent differential ability of PLCζ from disparate mammalian species to trigger Ca2+ oscillations in mouse oocytes. However, the molecular basis and confirmation of the apparent PLCζ species difference in activity remains to be provided. In the present study, we provide direct evidence for the superior effectiveness of human PLCζ relative to mouse PLCζ in generating Ca2+ oscillations in mouse oocytes. In addition, we have designed and constructed a series of human/mouse PLCζ chimeras to enable study of the potential role of discrete PLCζ domains in conferring the enhanced Ca2+ signalling potency of human PLCζ. Functional analysis of these human/mouse PLCζ domain chimeras suggests a novel role of the EF-hand domain in the species-specific differences in PLCζ activity. Our empirical observations are compatible with a basic mathematical model for the Ca2+ dependence of generating cytoplasmic Ca2+ oscillations in mammalian oocytes by sperm PLCζ.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Biosciences
Subjects:	R Medicine > R Medicine (General)
Publisher:	Oxford University Press
ISSN:	1360-9947
Funders:	Wellcome Trust
Date of Acceptance:	24 January 2014
Last Modified:	24 Feb 2024 02:08
URI:	https://orca.cardiff.ac.uk/id/eprint/79253

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