Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Secreted factors from ventral telencephalon induce the differentiation of GABAergic neurons in cortical cultures

Trinh, H.-h., Reid, J., Shin, Eunju ORCID: https://orcid.org/0000-0002-8865-6834, Liapi, A., Parnavelas, J. G. and Nadarajah, B. 2006. Secreted factors from ventral telencephalon induce the differentiation of GABAergic neurons in cortical cultures. European Journal of Neuroscience 24 (11) , pp. 2967-2977. 10.1111/j.1460-9568.2006.05194.x

[thumbnail of Trinh, 2006, EJN, Secreted factors from ventral forebrain induce differentiation of GABA neurons.pdf]
Preview
PDF - Published Version
Download (932kB) | Preview

Abstract

It is widely believed that the pyramidal cells and interneurons of the cerebral cortex are distinct in their origin, lineage and genetic make up. In view of these findings, the current thesis is that the phenotype determination of cortical neurons is primarily directed by genetic mechanisms. Using in vitro assays, the present study demonstrates that secreted factors from ganglionic eminence (GE) of the ventral telencephalon have the potency to induce the differentiation of a subset of cortical neurons towards c-aminobutyric acid (GABA)ergic lineage. Characterization of cortical cultures that were exposed to medium derived from GE illustrated a significant increase in the number of GABA-, calretinin- and calbindin-positive neurons. Calcium imaging together with pharmacological studies showed that the application of exogenous medium significantly elevated the intracellular calcium transients in cortical neurons through the activation of ionotropic glutamate receptors. The increase in GABA+ neurons appeared to be associated with the elevated calcium activity; treatment with blockers specific for glutamate receptors abolished both the synchronized transients and reduced the differentiation of GABAergic neurons. Such studies demonstrate that although intrinsic mechanisms determine the fate of cortical interneurons, extrinsic factors have the potency to influence their neurochemical differentiation and contribute towards their molecular diversity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
ISSN: 0953-816X
Date of First Compliant Deposit: 30 March 2016
Last Modified: 03 May 2023 21:51
URI: https://orca.cardiff.ac.uk/id/eprint/81021

Citation Data

Cited 6 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics