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The micromechanics of the superficial zone of articular cartilage

Mansfield, J. C., Bell, James Stephen ORCID: and Winlove, C. P. 2015. The micromechanics of the superficial zone of articular cartilage. Osteoarthritis and Cartilage 23 (10) , pp. 1806-1816. 10.1016/j.joca.2015.05.030

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Objective To investigate the relationships between the unique mechanical and structural properties of the superficial zone of articular cartilage on the microscopic scale. Design Fresh unstained equine metacarpophalangeal cartilage samples were mounted on tensile and compressive loading rigs on the stage of a multiphoton microscope. Sequential image stacks were acquired under incremental loads together with simultaneous measurements of the applied stress and strain. Second harmonic generation was used to visualise the collagen fibre network, while two photon fluorescence was used to visualise elastin fibres and cells. The changes visualised by each modality were tracked between successive loads. Results The deformation of the cartilage matrix was heterogeneous on the microscopic length scale. This was evident from local strain maps, which showed shearing between different regions of collagen under tensile strain, corrugations in the articular surface at higher tensile strains and a non-uniform distribution of compressive strain in the axial direction. Chondrocytes elongated and rotated under tensile strain and were compressed in the axial direction under compressive load. The magnitude of deformation varied between cells, indicating differences in either load transmission through the matrix or the mechanical properties of individual cells. Under tensile loading the reorganisation of the elastin network differed from a homogeneous elastic response, indicating that it forms a functional structure. Conclusions This study highlights the complexity of superficial zone mechanics and demonstrates that the response of the collagen matrix, elastin fibres and chondrocytes are all heterogeneous on the microscopic scale.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Uncontrolled Keywords: Second harmonic generation; Two photon fluorescence; Articular cartilage; Micromechanics; Elastin
Publisher: Elsevier
ISSN: 1063-4584
Date of Acceptance: 26 May 2015
Last Modified: 31 Oct 2022 09:42

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