Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Myosin-V regulates oskar mRNA localization in the drosophila oocyte

Krauss, J., Lopez De Quinto, Sonia ORCID:, Nusslein-Volhard, C. and Ephrussi, A. 2009. Myosin-V regulates oskar mRNA localization in the drosophila oocyte. Current Biology 19 (12) , pp. 1058-1063. 10.1016/j.cub.2009.04.062

Full text not available from this repository.


Intracellular mRNA localization is an effective mechanism for protein targeting leading to functional polarization of the cell. The mechanisms controlling mRNA localization and specifically how the actin and microtubule (MT) cytoskeletons cooperate in this process are not well understood. In Drosophila, Oskar protein accumulation at the posterior pole of the oocyte is required for embryonic development 1, 2, 3 and 4 and is achieved by the transport of oskar mRNA and its exclusive translation at the posterior pole 1, 5, 6 and 7. oskar mRNA localization requires the activity of the MT-based motor Kinesin [8], as well as the formation of a transport-competent ribonucleoprotein (RNP) complex [9]. Here, we show that didum, encoding the Drosophila actin-based motor Myosin-V 10 and 11, is a new posterior group gene that promotes posterior accumulation of Oskar. Myosin-V associates with the oskar mRNA transport complex preferentially at the oocyte cortex, revealing a short-range actomyosin-based mechanism that mediates the local entrapment of oskar at the posterior pole. Our results also show that Myosin-V interacts with Kinesin heavy chain and counterbalances Kinesin function, preventing ectopic accumulation of oskar in the cytoplasm. Our findings reveal that a balance of microtubule- and actin-based motor activities regulates oskar mRNA localization in the Drosophila oocyte.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0960-9822
Funders: RCUK
Last Modified: 17 Oct 2022 10:43

Citation Data

Cited 72 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item