Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Pericellular colocalisation and interactive properties of type VI collagen and perlecan in the intervertebral disc

Hayes, Anthony Joseph, Shu, C. C., Lord, M. S., Little, C. B., Whitelock, J. M. and Melrose, J. 2016. Pericellular colocalisation and interactive properties of type VI collagen and perlecan in the intervertebral disc. European Cells and Materials 32 , pp. 40-57. 10.22203/eCM.v032a03

[thumbnail of v032a03.pdf]
PDF - Published Version
Download (1MB) | Preview


The aim of this study was to immunolocalise type VI collagen and perlecan and determine their interactive properties in the intervertebral disc (IVD). Confocal laser scanning microscopy co-localised perlecan with type VI collagen as pericellular components of IVD cells and translamellar cross-bridges in ovine and murine IVDs. These cross-bridges were significantly less abundant in the heparin sulphate deficient Hspg2 exon 3 null mouse IVD than in wild type. This association of type VI collagen with elastic components provides clues as to its roles in conveying elastic recoil properties to annular tissues. Perlecan and type VI collagen were highly interactive in plasmon resonance studies. Pericellular colocalisation of perlecan and type VI collagen provides matrix stabilisation and cell-matrix communication which allows IVD cells to perceive and respond to perturbations in their biomechanical microenvironment. Perlecan, at the cell surface, provides an adhesive interface between the cell and its surrounding extracellular matrix. Elastic microfibrillar structures regulate tensional connective tissue development and function. The 2010 Global Burden of Disease study examined 291 disorders and identified disc degeneration and associated low back pain as the leading global musculoskeletal disorder emphasising its massive socioeconomic impact and the need for more effective treatment strategies. A greater understanding of how the IVD achieves its unique biomechanical functional properties is of great importance in the development of such therapeutic measures.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Uncontrolled Keywords: Perlecan, type VI collagen, pericellular matrix, extracellular matrix, mechanosensation, mechanotransduction, matrix stabilisation, translamellar cross-bridge.
Publisher: European Cells & Materials Ltd
ISSN: 1473-2262
Date of First Compliant Deposit: 12 July 2016
Date of Acceptance: 6 June 2016
Last Modified: 04 May 2023 20:59

Citation Data

Cited 25 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics