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Phospholipid analysis as a tool to study complex microbial communities in marine sediments

Rütters, Heike, Sass, Henrik ORCID:, Cypionka, Heribert and Rullkötter, Jürgen 2002. Phospholipid analysis as a tool to study complex microbial communities in marine sediments. Journal of Microbiological Methods 48 (2-3) , pp. 149-160. 10.1016/S0167-7012(01)00319-0

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To complement information on microbial communities in marine sediments that can be obtained using microbiological methods, we developed an analytical procedure to trace microbial lipids in environmental samples. We focused on analyzing intact phospholipids as these membrane constituents are known to be biomarkers for viable cells. Analysis of intact phospholipids from a fractionated and preconcentrated sediment extract was achieved using liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The combined analysis of phospholipid types and their fatty acid substituents allowed a differentiation between various groups of microorganisms living in the sediment. For comparison three strains of marine sulfate-reducing bacteria (SRB) were analysed for their lipid content.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Earth and Environmental Sciences
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Uncontrolled Keywords: HPLC-ESI-MS; Marine sediments; Microbial community structure; Phospholipids; Sulfate-reducing bacteria
Publisher: Elsevier
ISSN: 0167-7012
Last Modified: 18 Oct 2022 12:19

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