Rütters, Heike, Sass, Henrik ![]() |
Abstract
To complement information on microbial communities in marine sediments that can be obtained using microbiological methods, we developed an analytical procedure to trace microbial lipids in environmental samples. We focused on analyzing intact phospholipids as these membrane constituents are known to be biomarkers for viable cells. Analysis of intact phospholipids from a fractionated and preconcentrated sediment extract was achieved using liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The combined analysis of phospholipid types and their fatty acid substituents allowed a differentiation between various groups of microorganisms living in the sediment. For comparison three strains of marine sulfate-reducing bacteria (SRB) were analysed for their lipid content.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Earth and Environmental Sciences |
Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology |
Uncontrolled Keywords: | HPLC-ESI-MS; Marine sediments; Microbial community structure; Phospholipids; Sulfate-reducing bacteria |
Publisher: | Elsevier |
ISSN: | 0167-7012 |
Last Modified: | 18 Oct 2022 12:19 |
URI: | https://orca.cardiff.ac.uk/id/eprint/9315 |
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