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Gene amplification and qRT-PCR

Jones, Cerith and Filloux, Alain 2014. Gene amplification and qRT-PCR. In: Filloux, Alain and Ramos, Juan-Luis eds. Pseudomonas Methods and Protocols, Vol. 1149. Methods in Molecular Biology, Springer, pp. 457-468. (10.1007/978-1-4939-0473-0_35)

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This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell lysate from Pseudomonas species for colony PCR reactions. The chapter continues with a switch in focus from DNA to RNA, describing methods for RNA isolation from Pseudomonas, cDNA generation, and finally q-RT-PCR to investigate relative changes in gene expression.

Item Type: Book Section
Date Type: Published Online
Status: Published
Schools: Biosciences
Publisher: Springer
ISBN: 9781493904723
ISSN: 1064-3745
Last Modified: 06 Mar 2020 15:56

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