Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Global genome nucleotide excision repair is organized into domains that promote efficient DNA repair in chromatin

Yu, Shirong, Evans, Katie, Van Eijk, Patrick ORCID:, Bennett, Mark, Webster, Richard M., Leadbitter, Matthew, Teng, Yummin, Waters, Raymond, Jackson, Stephen P. and Reed, Simon H. ORCID: 2016. Global genome nucleotide excision repair is organized into domains that promote efficient DNA repair in chromatin. Genome Research 26 , pp. 1376-1387. 10.1101/gr.209106.116

[thumbnail of Genome Res.-2016-Yu-1376-87.pdf]
PDF - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview


The rates at which lesions are removed by DNA repair can vary widely throughout the genome, with important implications for genomic stability. To study this, we measured the distribution of nucleotide excision repair (NER) rates for UV-induced lesions throughout the budding yeast genome. By plotting these repair rates in relation to genes and their associated flanking sequences, we reveal that, in normal cells, genomic repair rates display a distinctive pattern, suggesting that DNA repair is highly organized within the genome. Furthermore, by comparing genome-wide DNA repair rates in wild-type cells and cells defective in the global genome–NER (GG-NER) subpathway, we establish how this alters the distribution of NER rates throughout the genome. We also examined the genomic locations of GG-NER factor binding to chromatin before and after UV irradiation, revealing that GG-NER is organized and initiated from specific genomic locations. At these sites, chromatin occupancy of the histone acetyl-transferase Gcn5 is controlled by the GG-NER complex, which regulates histone H3 acetylation and chromatin structure, thereby promoting efficient DNA repair of UV-induced lesions. Chromatin remodeling during the GG-NER process is therefore organized into these genomic domains. Importantly, loss of Gcn5 significantly alters the genomic distribution of NER rates; this has implications for the effects of chromatin modifiers on the distribution of mutations that arise throughout the genome.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: This article is available under a Creative Commons License (Attribution 4.0 International)
Publisher: Cold Spring Harbor Laboratory Press
ISSN: 1088-9051
Funders: MRC
Date of First Compliant Deposit: 17 October 2016
Date of Acceptance: 27 July 2016
Last Modified: 01 Nov 2022 11:33

Citation Data

Cited 17 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics