Chen, Shengxi, Fahmi, Nour Eddine, Nangreave, Ryan C., Mehellou, Youcef ![]() |
Official URL: http://dx.doi.org/10.1016/j.bmc.2012.02.024
Abstract
N,S-diprotected l-thiothreonine and l-allo-thiothreonine derivatives were synthesized using a novel chemical strategy, and used for esterification of the dinucleotide pdCpA. The aminoacylated dinucleotides were then employed for the preparation of activated suppressor tRNACUA transcripts. Thiothreonine and allo-thiothreonine were incorporated into a predetermined position of a catalytically competent dihydrofolate reductase (DHFR) analogue lacking cysteine, and the elaborated proteins were derivatized site-specifically at the thiothreonine residue with a fluorophore.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Pharmacy |
Subjects: | R Medicine > RM Therapeutics. Pharmacology |
Uncontrolled Keywords: | Sulfur-containing amino acids; Aminoacylation; Protein synthesis; Fluorescence labeling |
Publisher: | Elsevier |
ISSN: | 0968-0896 |
Last Modified: | 02 Nov 2022 10:03 |
URI: | https://orca.cardiff.ac.uk/id/eprint/97276 |
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