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ShenLingLan influences the attachment and migration of ovarian cancer cells potentially through the GSK3 pathway

Owen, Sioned, Ruge, Fiona, Gao, Yunong, Yang, Ying, Hou, Jianqing, Chen, Jian, Feng, Yi ORCID:, Zhang, Huiming, Wei, Cong, Wu, Yiling and Jiang, Wen Guo ORCID: 2017. ShenLingLan influences the attachment and migration of ovarian cancer cells potentially through the GSK3 pathway. Medicines 7 , pp. 1-11.

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Background: Ovarian cancer presents a major clinical challenge in the UK. Glycogen synthase kinase-3 (GSK-3) has been linked to cancer. This study tested the impact of ShenLingLan (SLDM) on ovarian cancer cell behaviour and its links to GSK-3. Methods: Fresh ovarian tumours (n = 52) were collected and processed. Histopathologcial and clinical information were collected and analysed against GSK-3 transcript levels using quantitative PCR (qPCR). Immortalised ovarian cancer cells’ protein alterations in response to SLDM were identified using a Kinexus™ protein kinase array. The effects of SLDM and a combination of SLDM and TWS119 on ovarian cancer cells ability to attach and migrate were evaluated using electrical cell-substrate impedance sensing (ECIS). Results: Transcript expression of GSK-3β was significantly increased in ovarian tumours which were poorly differentiated, patients with recurrence and in patients who had died from ovarian cancer. Treating SKOV-3 ovarian cells with SLDM reduced GSK-3 expression and GSK-3α (Y279). Treatment with SLDM reduced ovarian cancer cells ability to attach and migrate, which was further reduced in the presence of TWS119. Conclusions: This study identified a potential mechanism by which SLDM may exert anti-metastatic effects. Further work is needed to investigate the in vivo effects SLDM has on ovarian tumours.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords: ShenLingLan; ovarian cancer; cell migration; GSK3
Publisher: MDPI Publishing
Funders: Welsh National Research Network/Ser Cymru, Cancer Research Wales, the Cardiff University China Medical Scholarship
Date of First Compliant Deposit: 21 February 2017
Date of Acceptance: 15 February 2017
Last Modified: 19 Nov 2023 15:57

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