ShenLingLan influences the attachment and migration of ovarian cancer cells potentially through the GSK3 pathway

Owen, Sioned, Ruge, Fiona, Gao, Yunong, Yang, Ying, Hou, Jianqing, Chen, Jian, Feng, Yi ORCID: https://orcid.org/0000-0002-2445-1290, Zhang, Huiming, Wei, Cong, Wu, Yiling and Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111 2017. ShenLingLan influences the attachment and migration of ovarian cancer cells potentially through the GSK3 pathway. Medicines 7 , pp. 1-11.

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Abstract

Background: Ovarian cancer presents a major clinical challenge in the UK. Glycogen synthase kinase-3 (GSK-3) has been linked to cancer. This study tested the impact of ShenLingLan (SLDM) on ovarian cancer cell behaviour and its links to GSK-3. Methods: Fresh ovarian tumours (n = 52) were collected and processed. Histopathologcial and clinical information were collected and analysed against GSK-3 transcript levels using quantitative PCR (qPCR). Immortalised ovarian cancer cells’ protein alterations in response to SLDM were identified using a Kinexus™ protein kinase array. The effects of SLDM and a combination of SLDM and TWS119 on ovarian cancer cells ability to attach and migrate were evaluated using electrical cell-substrate impedance sensing (ECIS). Results: Transcript expression of GSK-3β was significantly increased in ovarian tumours which were poorly differentiated, patients with recurrence and in patients who had died from ovarian cancer. Treating SKOV-3 ovarian cells with SLDM reduced GSK-3 expression and GSK-3α (Y279). Treatment with SLDM reduced ovarian cancer cells ability to attach and migrate, which was further reduced in the presence of TWS119. Conclusions: This study identified a potential mechanism by which SLDM may exert anti-metastatic effects. Further work is needed to investigate the in vivo effects SLDM has on ovarian tumours.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords:	ShenLingLan; ovarian cancer; cell migration; GSK3
Publisher:	MDPI Publishing
Funders:	Welsh National Research Network/Ser Cymru, Cancer Research Wales, the Cardiff University China Medical Scholarship
Date of First Compliant Deposit:	21 February 2017
Date of Acceptance:	15 February 2017
Last Modified:	19 Nov 2023 15:57
URI:	https://orca.cardiff.ac.uk/id/eprint/98443

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