Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells

Hänggi, Pascal, Telezhkin, Vsevolod ORCID:, Kemp, Paul J. ORCID:, Schmugge, Markus, Gassmann, Max, Goede, Jeroen S., Speer, Oliver and Bogdanova, Anna 2015. Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells. American Journal of Physiology - Cell Physiology 308 (12) , C993-C1007. 10.1152/ajpcell.00395.2014

[thumbnail of ajpcell.00395.2014.pdf]
PDF - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview


Calcium signaling is essential to support erythroid proliferation and differentiation. Precise control of the intracellular Ca2+ levels in erythroid precursor cells (EPCs) is afforded by coordinated expression and function of several cation channels, including the recently identified N-methyl-d-aspartate receptor (NMDAR). Here, we characterized the changes in Ca2+ uptake and electric currents mediated by the NMDARs occurring during EPC differentiation using flow cytometry and patch clamp. During erythropoietic maturation, subunit composition and properties of the receptor changed; in proerythroblasts and basophilic erythroblasts, fast deactivating currents with high amplitudes were mediated by the GluN2A subunit-dominated receptors, while at the polychromatic and orthochromatic erythroblast stages, the GluN2C subunit was getting more abundant, overriding the expression of GluN2A. At these stages, the currents mediated by the NMDARs carried the features characteristic of the GluN2C-containing receptors, such as prolonged decay time and lower conductance. Kinetics of this switch in NMDAR properties and abundance varied markedly from donor to donor. Despite this variability, NMDARs were essential for survival of EPCs in any subject tested. Our findings indicate that NMDARs have a dual role during erythropoiesis, supporting survival of polychromatic erythroblasts and contributing to the Ca2+ homeostasis from the orthochromatic erythroblast stage to circulating red blood cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: American Physiological Society
ISSN: 0363-6143
Date of First Compliant Deposit: 12 March 2019
Date of Acceptance: 17 March 2015
Last Modified: 05 May 2023 17:52

Citation Data

Cited 8 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics