Hao, Chengcheng, Cui, Yuxin, Hu, M., Zhi, X., Zhang, Lijian, Li, Wenbin, Wu, Wei, Cheng, Shan and Jiang, Wen Guo ![]() |
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Abstract
Background: Osteopontin (OPN) is known to be involved in the development of certain cancers, including non-small cell lung cancer (NSCLC). However, its role in tumour progression remains unclear. The present study investigated the expression and biological impact of the OPN variant, OPN-a in NSCLC. Materials and Methods: OPN-a splicing variant expression in human NSCLC tissues was analyzed by real-time qPCR and immunohistochemistry (IHC), respectively. The impact of OPN-a on cellular functions of lung cancer cells was also evaluated. In addition, an in vitro model was developed for the assessment of interactions between lung cancer cells and bone tissue. Results: The expression of OPN-a was higher in lung cancer tissues compared to normal controls. OPN-a promoted the malignant phenotypes of A549 cells by enhancing cell-adherent abilities to bone tissues, which could be mediated by the interaction with the cell surface receptor αvβ3 integrin. Conclusion: OPN-a may represent a bone metastatic factor in human lung cancer, as well as a potential therapy target.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Subjects: | R Medicine > R Medicine (General) |
Publisher: | International Institute of Anticancer Research |
ISSN: | 0250-7005 |
Funders: | Cancer Research Wales, Cardiff China Medical Scholarship |
Date of First Compliant Deposit: | 29 January 2018 |
Date of Acceptance: | 5 April 2017 |
Last Modified: | 03 May 2023 17:46 |
URI: | https://orca.cardiff.ac.uk/id/eprint/100480 |
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