Hao, Chengcheng, Cui, Yuxin, Hu, M., Zhi, X., Zhang, Lijian, Li, Wenbin, Wu, Wei, Cheng, Shan and Jiang, Wen Guo  ORCID: https://orcid.org/0000-0002-3283-1111
2017.
OPN-a splicing variant expression in non-small cell lung cancer and its effects on the bone metastatic abilities of lung cancer cells in vitro.
Anticancer Research
37
(5)
, pp. 2245-2254.
10.21873/anticanres.11561
|
Preview |
PDF
- Published Version
Download (4MB) | Preview |
Abstract
Background: Osteopontin (OPN) is known to be involved in the development of certain cancers, including non-small cell lung cancer (NSCLC). However, its role in tumour progression remains unclear. The present study investigated the expression and biological impact of the OPN variant, OPN-a in NSCLC. Materials and Methods: OPN-a splicing variant expression in human NSCLC tissues was analyzed by real-time qPCR and immunohistochemistry (IHC), respectively. The impact of OPN-a on cellular functions of lung cancer cells was also evaluated. In addition, an in vitro model was developed for the assessment of interactions between lung cancer cells and bone tissue. Results: The expression of OPN-a was higher in lung cancer tissues compared to normal controls. OPN-a promoted the malignant phenotypes of A549 cells by enhancing cell-adherent abilities to bone tissues, which could be mediated by the interaction with the cell surface receptor αvβ3 integrin. Conclusion: OPN-a may represent a bone metastatic factor in human lung cancer, as well as a potential therapy target.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) |
| Publisher: | International Institute of Anticancer Research |
| ISSN: | 0250-7005 |
| Funders: | Cancer Research Wales, Cardiff China Medical Scholarship |
| Date of First Compliant Deposit: | 29 January 2018 |
| Date of Acceptance: | 5 April 2017 |
| Last Modified: | 03 May 2023 17:46 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/100480 |
Citation Data
Cited 13 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Dimensions
Dimensions