Freeley, Mark, Worthy, Harley L., Ahmed, Rochelle, Bowen, Ben, Watkins, Daniel, MacDonald, J. Emyr  ORCID: https://orcid.org/0000-0001-5504-1692, Zheng, Ming, Jones, D. Dafydd ORCID: https://orcid.org/0000-0001-7709-3995 and Palma, Matteo
2017.
Site-specific one-to-one click coupling of single proteins to individual carbon nanotubes: a single-molecule approach.
Journal of the American Chemical Society
139
(49)
, pp. 17834-17840.
10.1021/jacs.7b07362
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Abstract
We report the site-specific coupling of single proteins to individual carbon nanotubes (CNTs) in solution and with singlemolecule control. Using an orthogonal Click reaction, Green Fluorescent Protein (GFP) was engineered to contain a genetically encoded azide group and then bound to CNT ends in different configurations: in close proximity or at longer distances from the GFP’s functional centre. Atomic force microscopy and fluorescence analysis in solution and on surfaces at the single-protein level confirmed the importance of bioengineering optimal protein attachment sites to achieve direct protein-nanotube communication and bridging.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Physics and Astronomy Schools > Biosciences |
| Subjects: | Q Science > QC Physics |
| Publisher: | American Chemical Society |
| ISSN: | 0002-7863 |
| Date of First Compliant Deposit: | 22 November 2017 |
| Date of Acceptance: | 16 November 2017 |
| Last Modified: | 12 Nov 2024 01:00 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/106939 |
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