Site-specific one-to-one click coupling of single proteins to individual carbon nanotubes: a single-molecule approach

Freeley, Mark, Worthy, Harley L., Ahmed, Rochelle, Bowen, Ben, Watkins, Daniel, MacDonald, J. Emyr ORCID: https://orcid.org/0000-0001-5504-1692, Zheng, Ming, Jones, D. Dafydd ORCID: https://orcid.org/0000-0001-7709-3995 and Palma, Matteo 2017. Site-specific one-to-one click coupling of single proteins to individual carbon nanotubes: a single-molecule approach. Journal of the American Chemical Society 139 (49) , pp. 17834-17840. 10.1021/jacs.7b07362

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Abstract

We report the site-specific coupling of single proteins to individual carbon nanotubes (CNTs) in solution and with singlemolecule control. Using an orthogonal Click reaction, Green Fluorescent Protein (GFP) was engineered to contain a genetically encoded azide group and then bound to CNT ends in different configurations: in close proximity or at longer distances from the GFP’s functional centre. Atomic force microscopy and fluorescence analysis in solution and on surfaces at the single-protein level confirmed the importance of bioengineering optimal protein attachment sites to achieve direct protein-nanotube communication and bridging.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Physics and Astronomy Biosciences
Subjects:	Q Science > QC Physics
Publisher:	American Chemical Society
ISSN:	0002-7863
Date of First Compliant Deposit:	22 November 2017
Date of Acceptance:	16 November 2017
Last Modified:	12 Nov 2024 01:00
URI:	https://orca.cardiff.ac.uk/id/eprint/106939

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