Isolation and culture of adult intestinal, gastric, and lver organoids for cre-recombinase-mediated gene deletion

Flanagan, Dustin J., Schwab, Renate H. M., Tran, Bang M., Phesse, Toby J. ORCID: https://orcid.org/0000-0001-9568-4916 and Vincan, Elizabeth 2016. Isolation and culture of adult intestinal, gastric, and lver organoids for cre-recombinase-mediated gene deletion. Methods in Molecular Biology, Humana Press, pp. 1-11. (10.1007/7651_2016_14)

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Abstract

The discovery of Lgr5 as a marker of adult stem cells meant that stem cell populations could be purified and studied in isolation. Importantly, when cultured under the appropriate conditions these stem cells form organoids in tissue culture that retain many features of the tissue of origin. The organoid cultures are accessible to genetic and biochemical manipulation, bridging the gap between in vivo mouse models and conventional tissue culture. Here we describe robust protocols to establish organoids from gastrointestinal tissues (stomach, intestine, liver) and Cre-recombinase mediated gene manipulation in vitro.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Publisher:	Humana Press
ISSN:	10643745
Date of First Compliant Deposit:	23 March 2018
Date of Acceptance:	22 March 2018
Last Modified:	23 Oct 2022 13:15
URI:	https://orca.cardiff.ac.uk/id/eprint/110121

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