Brenchley, J. M., Hill, B. J., Ambrozak, D. R., Price, David  ORCID: https://orcid.org/0000-0001-9416-2737, Guenaga, F. J., Casazza, J. P., Kuruppu, J., Yazdani, J., Migueles, S. A., Connors, M., Roederer, M., Douek, D. C. and Koup, R. A.
2004.
T-cell subsets that harbor human immunodeficiency virus (HIV) in vivo: implications for HIV pathogenesis.
Journal of Virology
78
(3)
, pp. 1160-1168.
10.1128/JVI.78.3.1160-1168.2004
|
Preview |
PDF
- Published Version
Download (2MB) | Preview |
Abstract
Identification of T-cell subsets that are infected in vivo is essential to understanding the pathogenesis of human immunodeficiency virus (HIV) disease; however, this goal has been beset with technical challenges. Here, we used polychromatic flow cytometry to sort multiple T-cell subsets to 99.8% purity, followed by quantitative PCR to quantify HIV gag DNA directly ex vivo. We show that resting memory CD4+ T cells are the predominantly infected cells but that terminally differentiated memory CD4+ T cells contain 10-fold fewer copies of HIV DNA. Memory CD8+ T cells can also be infected upon upregulation of CD4; however, this is infrequent and HIV-specific CD8+ T cells are not infected preferentially. Naïve CD4+ T-cell infection is rare and principally confined to those peripheral T cells that have proliferated. Furthermore, the virus is essentially absent from naïve CD8+ T cells, suggesting that the thymus is not a major source of HIV-infected T cells in the periphery. These data illuminate the underlying mechanisms that distort T-cell homeostasis in HIV infection.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) |
| Publisher: | American Society for Microbiology |
| ISSN: | 0022-538X |
| Date of First Compliant Deposit: | 23 March 2018 |
| Date of Acceptance: | 7 October 2003 |
| Last Modified: | 05 May 2023 06:46 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/110127 |
Citation Data
Cited 305 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Altmetric
Altmetric