Role of hyaluronan in human adipogenesis: Evidence from in-vitro and in-vivo studies

Wilson, Nicholas, Steadman, Robert ORCID: https://orcid.org/0000-0002-1303-2496, Muller, Ilaria ORCID: https://orcid.org/0000-0003-2926-0722, Draman, Mohd, Rees, D. Aled ORCID: https://orcid.org/0000-0002-1165-9092, Taylor, Peter ORCID: https://orcid.org/0000-0002-3436-422X, Dayan, Colin M. ORCID: https://orcid.org/0000-0002-6557-3462, Ludgate, Marian and Zhang, Lei ORCID: https://orcid.org/0000-0003-3536-8692 2019. Role of hyaluronan in human adipogenesis: Evidence from in-vitro and in-vivo studies. International Journal of Molecular Sciences 20 (11) , 2675. 10.3390/ijms20112675

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Abstract

Hyaluronan (HA), an extra-cellular matrix glycosaminoglycan, may play a role in mesenchymal stem cell differentiation to fat but results using murine models and cell lines are conflicting. Our previous data, illustrating decreased HA production during human adipogenesis, suggested an inhibitory role. We have investigated the role of HA in adipogenesis and fat accumulation using human primary subcutaneous preadipocyte/fibroblasts (PFs, n = 12) and subjects of varying body mass index (BMI). The impact of HA on peroxisome proliferator-activated receptor gamma (PPARγ) expression was analysed following siRNA knockdown or HA synthase (HAS)1 and HAS2 overexpression. PFs were cultured in complete or adipogenic medium (ADM) with/without 4-methylumbelliferone (4-MU = HA synthesis inhibitor). Adipogenesis was evaluated using oil red O (ORO), counting adipogenic foci, and measurement of a terminal differentiation marker. Modulating HA production by HAS2 knockdown or overexpression increased (16%, p < 0.04) or decreased (30%, p = 0.01) PPARγ transcripts respectively. The inhibition of HA by 4-MU significantly enhanced ADM-induced adipogenesis with 1.52 ± 0.18- (ORO), 4.09 ± 0.63- (foci) and 2.6 ± 0.21-(marker)-fold increases compared with the controls, also increased PPARγ protein expression (40%, (p < 0.04)). In human subjects, circulating HA correlated negatively with BMI and triglycerides (r = −0.396 (p = 0.002), r = −0.269 (p = 0.038), respectively), confirming an inhibitory role of HA in human adipogenesis. Thus, enhancing HA action may provide a therapeutic target in obesity.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Publisher:	MDPI
ISSN:	1422-0067
Date of First Compliant Deposit:	3 June 2019
Date of Acceptance:	29 May 2019
Last Modified:	11 Oct 2023 17:11
URI:	https://orca.cardiff.ac.uk/id/eprint/123118

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