Emergence of mcr-1 mediated colistin resistant Escherichia coli from a hospitalized patient in Bangladesh

Farzana, Refath, Jones, Lim S, Rahman, Md. Anisur, Toleman, Mark A ORCID: https://orcid.org/0000-0002-9497-0512, Sands, Kirsty, Portal, Edward, Boostrom, Ian, Kalam, Md. Abul, Hassan, Brekhna, Uddin, AKM Nasir and Walsh, Timothy R ORCID: https://orcid.org/0000-0003-4315-4096 2019. Emergence of mcr-1 mediated colistin resistant Escherichia coli from a hospitalized patient in Bangladesh. Journal of Infection in Developing Countries 13 (08) , pp. 773-776. 10.3855/jidc.11541

Preview

PDF - Published Version Available under License Creative Commons Attribution. Download (1MB) | Preview

Abstract

Introduction: The emergence of plasmid mediated mcr in bacteria has become global public health threat. Herein, we report a mcr-1 positive E. coli in normal human flora from a patient admitted in Dhaka Medical College Hospital (DMCH). Methodology: In total, 700 non-duplicate rectal swabs were collected from DMCH during 13th May to 12th June 2018. E. coli from rectal swabs were isolated on chromogenic UTI media containing vancomycin 10mg/l (Liofilchem, Italy) and confirmed by MALDI-TOF. Minimum inhibitory concentrations (MIC) were determined by agar dilution and interpreted according to EUCAST breakpoints. Genomic analysis of mcr positive E. coli (MCRPEC) was performed by Illumina MiSeq sequencing and pulsed field gel electrophoresis (PFGE) using S1 nuclease DNA digests and blamcr-1 probing. Transferability of blamcr-1 were determined by conjugation assays. Results: We found one MCRPEC from 700 rectal swab screening which was isolated from the rectal swab culture of a 17-year boy who was admitted to the burns ICU, DMCH with 53% flame burn involving much of the trunk and face. Genome sequencing revealed that mcr-1 was present on an IncH12 plasmid of 257,243 bp and flanked by ISApaI1. The colistin resistance can be transferred to the recipient Klebsiella varricola with a frequency of 8.3 × 10-5. Transconjugants were more resistant to colistin than donor (MIC 32 µg/mL). Conclusions: This is the first human associated mcr in Bangladesh. These data indicate the need for a systematic “one health” surveillance in the country.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	J INFECTION DEVELOPING COUNTRI
ISSN:	1972-2680
Date of First Compliant Deposit:	10 October 2019
Date of Acceptance:	24 July 2019
Last Modified:	14 Mar 2024 02:07
URI:	https://orca.cardiff.ac.uk/id/eprint/125951

Citation Data

Cited 4 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item