A molecular basis for the T cell response in HLA-DQ2.2 mediated celiac disease

Ting, Yi Tian, Dahal-Koirala, Shiva, Kim, Hui Shi Keshia, Qiao, Shuo-Wang, Neumann, Ralf S., Lundin, Knut E. A., Petersen, Jan, Reid, Hugh H., Sollid, Ludvig M. and Rossjohn, Jamie ORCID: https://orcid.org/0000-0002-2020-7522 2020. A molecular basis for the T cell response in HLA-DQ2.2 mediated celiac disease. Proceedings of the National Academy of Sciences 117 (6) , pp. 3063-3073. 10.1073/pnas.1914308117

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Abstract

The highly homologous human leukocyte antigen (HLA)-DQ2 molecules, HLA-DQ2.5 and HLA-DQ2.2, are implicated in the pathogenesis of celiac disease (CeD) by presenting gluten peptides to CD4+ T cells. However, while HLA-DQ2.5 is strongly associated with disease, HLA-DQ2.2 is not, and the molecular basis underpinning this differential disease association is unresolved. We here provide structural evidence for how the single polymorphic residue (HLA-DQ2.5-Tyr22α and HLA-DQ2.2-Phe22α) accounts for HLA-DQ2.2 additionally requiring gluten epitopes possessing a serine at the P3 position of the peptide. In marked contrast to the biased T cell receptor (TCR) usage associated with HLA-DQ2.5–mediated CeD, we demonstrate with extensive single-cell sequencing that a diverse TCR repertoire enables recognition of the immunodominant HLA-DQ2.2-glut-L1 epitope. The crystal structure of two CeD patient-derived TCR in complex with HLA-DQ2.2 and DQ2.2-glut-L1 (PFSEQEQPV) revealed a docking strategy, and associated interatomic contacts, which was notably distinct from the structures of the TCR:HLA-DQ2.5:gliadin epitope complexes. Accordingly, while the molecular surfaces of the antigen-binding clefts of HLA-DQ2.5 and HLA-DQ2.2 are very similar, differences in the nature of the peptides presented translates to differences in responding T cell repertoires and the nature of engagement of the respective antigen-presenting molecules, which ultimately is associated with differing disease penetrance.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	National Academy of Sciences
ISSN:	0027-8424
Date of First Compliant Deposit:	12 March 2020
Date of Acceptance:	19 December 2019
Last Modified:	05 May 2023 22:10
URI:	https://orca.cardiff.ac.uk/id/eprint/130360

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