Accelerating biphasic biocatalysis through new process windows

Huynh, Florence, Tailby, Matthew, Finniear, Aled, Stephens, Kevin, Allemann, Rudolf K. ORCID: https://orcid.org/0000-0002-1323-8830 and Wirth, Thomas ORCID: https://orcid.org/0000-0002-8990-0667 2020. Accelerating biphasic biocatalysis through new process windows. Angewandte Chemie International Edition 59 (38) , pp. 16490-16495. 10.1002/anie.202005183

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Abstract

Process intensification through continuous flow reactions has increased the production rates of fine chemicals and pharmaceuticals. Catalytic reactions are accelerated through an unconventional and unprecedented use of a high‐performance liquid/liquid counter current chromatography system. Product generation is significantly faster than in traditional batch reactors or in segmented flow systems, which is exemplified through stereoselective phase‐transfer catalyzed reactions. This methodology also enables the intensification of biocatalysis as demonstrated in high yield esterifications and in the sesquiterpene cyclase‐catalyzed synthesis of sesquiterpenes from farnesyl diphosphate as high‐value natural products with applications in medicine, agriculture and the fragrance industry. Product release in sesquiterpene synthases is rate limiting due to the hydrophobic nature of sesquiterpenes, but a biphasic system exposed to centrifugal forces allows for highly efficient reactions.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Chemistry
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Publisher:	Wiley
ISSN:	1433-7851
Funders:	BioExtractions (Wales) and Knowledge Economy Skills Scholarships (KESS)
Date of First Compliant Deposit:	18 August 2020
Date of Acceptance:	22 June 2020
Last Modified:	08 May 2023 20:59
URI:	https://orca.cardiff.ac.uk/id/eprint/134268

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