Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons

Cook, Sophie R., Badell-Grau, Rafael A., Kirkham, Emily D., Jones, Kimberley M., Kelly, Brendan M., Winston, Jincy, Waller-Evans, Helen ORCID: https://orcid.org/0000-0003-4133-6064, Allen, Nicholas D. ORCID: https://orcid.org/0000-0003-4009-186X and Lloyd-Evans, Emyr ORCID: https://orcid.org/0000-0002-3626-1611 2020. Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons. AMRC Open Research 2 , 21. 10.12688/amrcopenres.12903.1

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Abstract

Good’s buffers are commonly used for cell culture and, although developed to have minimal to no biological impact, they cause alterations in cellular processes such as autophagy and lysosomal enzyme activity. Using Chinese hamster ovary cells and induced pluripotent stem cell-derived neurons, this study explores the effect of zwitterionic buffers, specifically HEPES, on lysosomal volume and Ca2+ levels. Certain zwitterionic buffers lead to lysosomal expansion and reduced lysosomal Ca2+. Care should be taken when selecting buffers for growth media to avoid detrimental impacts on lysosomal function.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
ISSN:	2517-6900
Date of First Compliant Deposit:	27 August 2020
Date of Acceptance:	5 May 2020
Last Modified:	06 Mar 2024 02:07
URI:	https://orca.cardiff.ac.uk/id/eprint/134538

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