Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation

Alahmadi, Alaa and Ramji, Dipak P. ORCID: https://orcid.org/0000-0002-6419-5578 2022. Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation. Ramji, Dipak, ed. Atherosclerosis: Methods and Protocols, Vol. 2419. Methods in Molecular Biology, New York, NY: Springer, pp. 247-255. (10.1007/978-1-0716-1924-7_14)

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Abstract

Macrophage foam cell formation plays a crucial role in the initiation and progression of atherosclerosis. Macrophages uptake native and modified low density lipoprotein (LDL) through either receptor-dependent or receptor-independent mechanisms to transform into lipid laden foam cells. Foam cells are involved in the formation of fatty streak that is seen during the early stages of atherosclerosis development and therefore represents a promising therapeutic target. Normal or modified lipoproteins labeled with fluorescent dyes such as 1,1′-dioctadecyl-3-3-3′,3′-tetramethylindocarbocyanine perchlorate (Dil) are often used to monitor their internalization during foam cell formation. In addition, the fluorescent dye Lucifer Yellow (LY) is widely used as a marker for macropinocytosis activity. In this chapter, we describe established methods for monitoring modified lipoprotein uptake and macropinocytosis during macrophage foam cell formation.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Springer
ISBN:	9781071619230
ISSN:	1064-3745
Last Modified:	10 Nov 2022 10:45
URI:	https://orca.cardiff.ac.uk/id/eprint/148035

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