Almotiri, Alhomidi, Abdelfattah, Ali and Rodrigues, Neil P.  ORCID: https://orcid.org/0000-0002-1925-7733
2022.
Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis.
Ramji, Dipak, ed.
Atherosclerosis,
Vol. 2419.
Methods in Molecular Biology,
Springer,
pp. 583-595.
(10.1007/978-1-0716-1924-7_36)
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Abstract
Advancing age causes physiologic decline in tissue function. In the hematopoietic system this manifests as a progressive reduction in blood or immune cell function and clonal hematopoiesis, where a mutated hematopoietic stem cell can dominate blood cell production and confer an increased propensity for myeloid malignancy. In the aging cardiovascular system, atherosclerosis causes an inflammatory cell- driven accumulation of lipid-derived plaques in major arteries which constrains blood flow and can lead to myocardial infarction and stroke. Clonal hematopoiesis in the elderly has recently been associated with a substantially increased risk of atherosclerosis-related cardiovascular disease. However, the direct association between deregulated hematopoiesis in clonal hematopoiesis and atherosclerosis is poorly defined. Herein, we describe a flow cytometry method to prospectively analyze the crucial hematopoietic stem/progenitor, inflammatory and lymphoid cell participants in atherosclerosis. This analysis can be applied to decipher the complex relationship between hematopoietic cell types involved in clonal hematopoiesis and atherosclerosis in mouse models.
| Item Type: | Book Section |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Biosciences Research Institutes & Centres > European Cancer Stem Cell Research Institute (ECSCRI) |
| Publisher: | Springer |
| ISBN: | 9781071619247 |
| ISSN: | 1064-3745 |
| Last Modified: | 22 Jun 2023 09:59 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/148114 |
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